rpmA [2019-07-08 10:31:49]

ribosomal protein

locus: BSU27940

pI: 10.76

mw: 10.23 kDa

protein length: 94 aa

gene length: 285 bp

function: translation

product: ribosomal protein L27 (BL24)

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,854,880 2,855,164

Phenotypes of a mutant

• essential [Pubmed|23002217,12682299]

The protein

Protein family

• bacterial [SW|ribosomal protein] bL27 family (according to UniProt)

Modification

• the N-terminal extension is cleaved off by [protein|B27548A4BBF396C9BA102A80ACB08B21262CEA30|Prp] [Pubmed|25388641]

Structure

• [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|RbgA] [Pubmed|24335279]

Expression and Regulation

Operons

• genes: [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]-[gene|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp]-[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • likely autorepression of the [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]→[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA] operon expression upon binding of excess of one ribosomal protein to the untranslated region of the [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]→[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA] mRNA [Pubmed|17616982]

• genes: [gene|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA]-[gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]-[gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]-[gene|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp]-[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]
  description: [Pubmed|22383849]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|15699190,1942049,15383836]

  additional information

  • the domains that inhibits [protein|search|SpoIVFB] is cleaved off by [protein|search|CtpB] (second cleavage) [PubMed|24243021]

• genes: [gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]
  description: [Pubmed|8969504]

References

• 19653700,8969504,23002217,24335279,25388641,22383849,25903689,28516784